Diabetes mellitus is a major chronic disease that predisposes an individual to severe health complications. The modes of action against diabetes involve inhibiting activities of enzymes such as alpha-amylase, alpha-glucosidase and suppression of oxidative stress emerging due to hyperglycemia. The conventional drugs used for treating diabetes may result in severe side effects. In wake of this, several studies are now exploring the option of using phytochemicals extracted from plants as an alternative method for treating diabetes. The current study investigated the anti-diabetic property of Aster (Callistephus chinensis) flower waste (AFW) extract using in-vitro assays such as alpha-amylase inhibitory assay and glucose uptake by yeast cells. Additionally, antioxidant activity of the extract was evaluated using ABTS assay. The IC50 of AFW extract in alpha-amylase inhibitory assay was found to be 1.37 mg/ml, which reflects its promising salivary alpha-amylase inhibitory activity. The glucose uptake assay revealed that 0.5mg/ml extract facilitated maximum increase in glucose uptake (96.875 %) by yeast cells. The rise in percent glucose uptake in yeast cells (91.303%) elicited in the presence of 2 mg/ml AFW extract and 25 mM glucose system was found to be comparable to that observed for 2 mg/ml standard metformin (92.307%). The IC50 value of AFW extract in ABTS assay was found to be 0.195 mg/ml, which corroborates its strong antioxidant property. In conclusion, in-vitro assays showed that AFW extract exhibits favorable in-vitro anti-diabetic and antioxidant properties. Our study attempted to highlight the use of organic flower waste for extracting valuable phytochemicals of therapeutic value.